. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 74 BARBARA BROWN McDONALD IV. Fixation and staining of individual cells Individual containers for the fixation of cells from small clones were prepared by sealing paraffin rings to slides. The rings were formed by dipping a silicone- coated vial (10 mm. outside diameter), filled with cold water, into molten paraffin, and then into cold water. The paraffin coat was cut into rings about 5 mm. deep, which were slipped off the vial and sealed to the slide by careful heating. Two. 400 450 500 550 600 Wavelength, m/j. 650 FIGURE 1
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 74 BARBARA BROWN McDONALD IV. Fixation and staining of individual cells Individual containers for the fixation of cells from small clones were prepared by sealing paraffin rings to slides. The rings were formed by dipping a silicone- coated vial (10 mm. outside diameter), filled with cold water, into molten paraffin, and then into cold water. The paraffin coat was cut into rings about 5 mm. deep, which were slipped off the vial and sealed to the slide by careful heating. Two. 400 450 500 550 600 Wavelength, m/j. 650 FIGURE 1. Absorption curve of a Tetrahymena macronucleus stained with Feulgen dye. The two wave-lengths (490 m/u and 514 m/t) used in making photometric measurements are indicated. were placed on each slide, directly above notched circles which had been drawn on the opposite side of the slide with a diamond pencil. The notches, facilitating the location of cells, could be seen at the inner edges of the paraffin rings. Fixation was carried out under two dissecting microscopes—one for the isola- tion of the cell, and one for its fixation. About ml. of fixative was placed in one of the paraffin containers. The cell was delivered carefully from a micro- pipette at the surface of the slide, and a coverslip was placed on top of the con-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
