Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . is best to keep the dye as asaturated alcoholic solution. For staining, however, alcoholic solutionsare of little value, as they do not seem to possess the penetration ofwatery solutions. The usual strength employed is a one to two percent, aqueous solution of the dye; the stains are unpleasant to handleand may be prepared as follows: To a test-tube—diameter 13 15 mm.—two-thirds full of distilled water add, drop by drop, asaturated alcoholic solution of the dye until the water i
Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . is best to keep the dye as asaturated alcoholic solution. For staining, however, alcoholic solutionsare of little value, as they do not seem to possess the penetration ofwatery solutions. The usual strength employed is a one to two percent, aqueous solution of the dye; the stains are unpleasant to handleand may be prepared as follows: To a test-tube—diameter 13 15 mm.—two-thirds full of distilled water add, drop by drop, asaturated alcoholic solution of the dye until the water is barely trans-parent. This is then poured into a bottle adapted with a Barnes dropper.(See cut of appropriate stain bottle, p. 46.) The solution so-preparedis about per cent., and may be used whenever a one or two per cent,solution of the dye is recommended. The addition of carbolic acid toaqueous solutions of some anilin dyes seems to enhance their stainingpower and keeping properties. For this reason, instead of plain distilledwater, as previously recommended, many workers use a five per
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