Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . s constructed essentially the sameas the pipettes which accompany the Thoma-Zeiss Apparatus1 see p. [82). The capillary portion, however, is graduated I,Yz and l/> which enables the observer to dilute the bloodsample in the proportion of 1 :200, 1 1300 or 1 1400 as he maydesire. If there is difficulty in drawing in the blood exactlyto one of the graduations just mentioned the amount of bloodabove or below the volume indicated by the graduation m
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . s constructed essentially the sameas the pipettes which accompany the Thoma-Zeiss Apparatus1 see p. [82). The capillary portion, however, is graduated I,Yz and l/> which enables the observer to dilute the bloodsample in the proportion of 1 :200, 1 1300 or 1 1400 as he maydesire. If there is difficulty in drawing in the blood exactlyto one of the graduations just mentioned the amount of bloodabove or below the volume indicated by the graduation maybe determined by means of certain delicate cross-lines whichare placed directly above and below the graduation. Eachcross-line corresponds to T^ of the volume of the capillarytube from the tip to the 1 graduation. A per cent solution of sodium carbonate is used to dis-solve the stroma of the erythrocytes and so render the bloodsolution perfectly clear. If this is not done the color of theblood solution invariably appears darker in tone than that ofthe colored glass wedge. A freshly prepared sodium carbo-•3 178 PHYSIOLOGICAL CHEMISTRY. nate solution should be used in order that the clearness ofthe solution may not be marred by the presence of sodiumbicarbonate. 3. Dares Haemoglobinometer (Fig. 67, below).—Thisinstrument, as the name signifies, is used for the determina-tion of haemoglobin. Inusing either Fleischlshremometer or the in-strument as modified byMiescher the blood is di-luted for examinationwhereas with the Dareinstrument no dilution isrequired. This probablyallows of rather moreaccurate determinationsthan are possible withthe old Fleischl appa-ratus. The instrument con-sists essentially of the fol-lowing parts : (1) A cap-illary observation cell,(2) a semicircular col-ored glass wedge, (3) amilled wheel for manip-ulating the wedge, (4)a candle used to illumi-nate portions of the cap-illary observation celland the colored wedge,(5) a small telescope used in
Size: 1551px × 1611px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, bookpublisherphiladelphiablakis
