. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 316 J. R. GI A. 1 ?D D T] 2 "" Z — Tl - n T1 31 f40 « "35 "u E o ,''' • 30 '5 ^ ' o u ,** OJ CL 0- 0* 03 "o 1 *" l^H f^K iJtlllf -20 < 4^ c at - 10 w £ - z 1 | I 1 1 1 1 1 1 1 1 I I T t I 1 1 1 I T I 1 1 ™ 1 1 1 IM 1 1 0 10 20 30 40 Fraction number Peak One Fraction 26 Peak Two Fraction 32 Peak Three Fraction 36 Peak Four Fraction 40 pQDPFLRFa Figure 5. Reverse phase HPLC of acidified methanol extracts of the prosomal CNS as assayed for FaRP-like bioactivity with the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 316 J. R. GI A. 1 ?D D T] 2 "" Z — Tl - n T1 31 f40 « "35 "u E o ,''' • 30 '5 ^ ' o u ,** OJ CL 0- 0* 03 "o 1 *" l^H f^K iJtlllf -20 < 4^ c at - 10 w £ - z 1 | I 1 1 1 1 1 1 1 1 I I T t I 1 1 1 I T I 1 1 ™ 1 1 1 IM 1 1 0 10 20 30 40 Fraction number Peak One Fraction 26 Peak Two Fraction 32 Peak Three Fraction 36 Peak Four Fraction 40 pQDPFLRFa Figure 5. Reverse phase HPLC of acidified methanol extracts of the prosomal CNS as assayed for FaRP-like bioactivity with the RPM of Biwvfoii A. composite HPLC profile of the third HPLC run. The HPLC gradient used and the elution times of standard FaRPs (a = amide) are shown. Four bioactive peaks were detected (black), and these fractions were assayed further. B. Contractures of the Busycnn RPM produced by peak fractions and by synthetic pQDPFLRFamide. TNRNFLRFamide and SDRNFLRFamide also increase cardiac ganglion burst rate, probably by a similar cellular mechanism. The positive inotropic actions of FaRPs on the Limulus heart are, at least in part, due to an enhance- ment of cardiac muscle contractility. However, the effect of these FaRPs on transmitter release at the cardiac neu- romuscular junction is not known. We have not failed to notice that the actions of TNRNFLRFamide and SDRNFLRFamide on the Limulus myocardium are sim- ilar to those produced by dopamine or proctolin (Watson and Groome. 1989). FaRPs might act via similar mech- anisms, or might induce the release of these modulators from cardiac ganglion neurons. Slnicliirc-iictivily relationship of synthetic FaRPs The Linmliis heart is responsive to several N-terminally extended FaRPs, whereas RNFLRFamide is less effective, and the tetrapeptides FLRFamide and FMRFamide are essentially inactive on this preparation. The relative po- D S 253 IQ2 o 1 1 59 % ' 9 2 0 ?S p 006- ? 21 0(M. 3 , nnfr fUfl fll [r, rHV Peak One 01
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
