. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 13. Stability of dogfish lens nuclear fiber cells after extraction with tris buffer (A), 8 A/ urea (B), 1% SDS (C), and 1% SDS plus 50 mM DTT. (D) 1% SDS plus 50 mA/ DTT for 24 h. The fibers are thinner and appear to disintegrate with time. Only SDS plus DTT was capable of totally disintegrating the fiber membranes. 1981). These structural alterations parallel the diminished elasticity of the nuclear fiber cells. On a chemical basis, one finds that there are two major elements of the cytoskeleton:vimentin and actin. A
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 13. Stability of dogfish lens nuclear fiber cells after extraction with tris buffer (A), 8 A/ urea (B), 1% SDS (C), and 1% SDS plus 50 mM DTT. (D) 1% SDS plus 50 mA/ DTT for 24 h. The fibers are thinner and appear to disintegrate with time. Only SDS plus DTT was capable of totally disintegrating the fiber membranes. 1981). These structural alterations parallel the diminished elasticity of the nuclear fiber cells. On a chemical basis, one finds that there are two major elements of the cytoskeleton:vimentin and actin. An SDS polyacrylamide gel of the lens fiber membrane-cytoskeletal complex reveals peptides at 55,000 and at 45,000 daltons, which represent vimentin (55,000) and actin (45,000). Figure 15 illustrates the SDS- polyacrylamide gel profile of skeletal and other membrane elements of cow lenses (Benedetti el al., 1981). The loss of intermediate filaments in the nucleus is. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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