. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. To Prepare Bouillon from Meat Extract 193 n. To Prepare Bouillon from Meat Extract.âWhen desirable, the bouillon may also be prepared from beef-extract, the method being very simple: To 1000 cc. of clean water 10 grams of beef-peptone, 5 grams of sodium chlorid, and about 2 grams of beef-extract are added. The solution is boiled until the constituents are dissolved, titrated, and filtered when cold. If it be filtered while hot, there is always a subseque
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. To Prepare Bouillon from Meat Extract 193 n. To Prepare Bouillon from Meat Extract.âWhen desirable, the bouillon may also be prepared from beef-extract, the method being very simple: To 1000 cc. of clean water 10 grams of beef-peptone, 5 grams of sodium chlorid, and about 2 grams of beef-extract are added. The solution is boiled until the constituents are dissolved, titrated, and filtered when cold. If it be filtered while hot, there is always a subsequent precipitation of pieat-salts, which clouds it. Bouillon and other liquid culture media are best dispensed and â kept in small receptaclesâtest-tubes or flasksâ^in order that a single contaminating organism, should it enter, may not spoil the entire. Kg- 37.âFunnel for filling tubes with culture media (Warren): a, Funnel containing the culture media in liquid condition; b, pinch-cock by which the floiy of fluid into the test-tube is regulated; c, rubber tubing. quantity. A convenient, simple apparatus for filling tubes with liquid media consists of a funnel to which a short glass pipet is at- tached by a bit of rubber tubing. A pinch-cock controls the outflow of the liquid. The apparatus need not be steriHzed before using, as the culture medium must subsequently be sterilized either by the intermittent method or in the autoclave after the tubes are filled. The test-tubes and flasks into which the culture medium is filled must, however, be previously plugged and sterilized by dry heat, unless the subsequent sterilization is to be performed in the auto- clave, when it may be unnecessary. 13. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original McFarland, Joseph, 1868-. Philadelphia and London, W. B. Saunders
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
