. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SULFUR METABOLISM IN ARENICOLA 359 individual standards of these compounds in the manner just described. Recoveries in excess of 95% were obtained in all cases when 15 standard samples were treated as just outlined. Thompson and Morris (1959) reported the chromatographic separation of mixtures of amino acids on a column of Dowex 50 (Na+), using water or aqueous ethanol as solvent. This suggested separation of acidic compounds from Arenicola by chromatography on a column of Dowex 50 (H+), using distilled water for elution. Th
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SULFUR METABOLISM IN ARENICOLA 359 individual standards of these compounds in the manner just described. Recoveries in excess of 95% were obtained in all cases when 15 standard samples were treated as just outlined. Thompson and Morris (1959) reported the chromatographic separation of mixtures of amino acids on a column of Dowex 50 (Na+), using water or aqueous ethanol as solvent. This suggested separation of acidic compounds from Arenicola by chromatography on a column of Dowex 50 (H+), using distilled water for elution. The column, 60 X cm., was prepared from a slurry of reagent grade Dowex 50 (H+) which had previously been washed to remove excess acidity. A column was used only once. The sample to be resolved was placed in the column in a volume of 1 ml. Elution was carried out at a rate of 5 to 6 ml. per hour. One-milliliter fractions were collected. Mixtures of standards could not be resolved until a soluble inorganic salt was added to the sample (25 to 50 NaCl), whereupon resolution became excellent (Fig. 1). The E > •£ TAURINE CYSTEIC ACID TAUROCYAMINE. CYSTEINE SULFINIC ACID 15 25 35 45 FRACTION NUMBER 55 65 FIGURE 1. Elution of standards from cm. Dowex 50 (H+) column. worm extracts contained sufficient inorganic material to obviate addition of salt. Schram and Crokaert (1957) obtained similar resolution of taurine and tauro- cyamine by eluting Dowex 50 (H+) columns with M, pH citrate buffer. The four acidic components could be resolved by one-dimensional paper chromatography with phenol: water (72% v/v) as solvent. The basic components were separated by two-dimensional paper chromatography with phenol: water and 2,4-lutidine: water (65% v/v) as solvents. Papers used were Whatman Nos. 1, 4, or 3 MM. Quantitative determinations of amino compounds were carried out by the method of Landua and Awapara (1949). Guanidylated entities were determine
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
