. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 2. RFLP genotypes of Svmhiinliiiiuin. The same srDNAs were amplified with host-excluding PCR primers (A) and with universal PCR primers (B). and then digested with Dpn II (upper panels) and with Taq I (lower panels). Clones are srDNA standards for genotype N (clone N°~l: see Results). Mtmlastraea annularis (clone H"), Symbiodinium B (clone B"}. SvmhiiiJinium C (clone C"), Svmbioiliniuni A (clone .4"). Symbio- dimiiin E (clone £""'), and Symbiodinium C2 (clone C2° '). Samples are zooxanthe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 2. RFLP genotypes of Svmhiinliiiiuin. The same srDNAs were amplified with host-excluding PCR primers (A) and with universal PCR primers (B). and then digested with Dpn II (upper panels) and with Taq I (lower panels). Clones are srDNA standards for genotype N (clone N°~l: see Results). Mtmlastraea annularis (clone H"), Symbiodinium B (clone B"}. SvmhiiiJinium C (clone C"), Svmbioiliniuni A (clone .4"). Symbio- dimiiin E (clone £""'), and Symbiodinium C2 (clone C2° '). Samples are zooxanthellae from Moniastraea scored as Symbiodinium C2 (B; C2). Symbioilinitim C2 and Symbititlinium E in approximately equal amounts (A and B; C2~E), more Symbiodinium A than Symbiodinium E (A and B; A > El. more S\mbiodinium E than Symbiodinium A (A and B; E > A), more Symbiodinium A than Symbiodinium C2 (A and B; A > C2). more Syinbimliniiim C2 than Symbiodinium A (A and B: C2 > A), and Symbio- iliiiium A and C2 in approximately equal amounts with more Symbiodinium E (A and B; E > A—C2). Lane M contains DNA fragment size standards of (top to bottom) 1500 base pairs (bp). 1200 bp. and then 1000 hp to 100 bp in 100-bp increments. below). Further observations on genotype N are presented elsewhere (Toller et til., in press). The srDNA genotypes of Symbiodinium A. B, C, and E are represented by cloned srDNAs A". B°. C". and E°~' (respectively), and these genotypes differ from one another in both Dpn II and Tat/ I digests (Toller et til., 2001; Fig. 2A. B). RFLP genotype C2. represented in Figure 2A by a cloned srDNA (C2"~': below) and in Figure 2B by a sample of zooxanthellae (C2), has not been found in unmanipulated colonies of Montastraea annularis and M. faveolata (Rowan and Knowlton. 1995; Rowan et al., 1997; Toller et til., 2001; this study and unpubl. obs.). Genotype C2 is distinguished from genotypes C and E only when both Dpn II and Taq I dig
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
