. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. 202 BACTERIOLOGY Fig. 37. hydroxide solution gives the proportion of CO2 absorbed. The explosive character of the residue is determined as follows: the thumb is placed again over the mouth of the bulb and the gas from the closed branch is allowed to flow into the bulb and mix with the air there present. The plug is then removed and a lighted match inserted into the mouth of the bulb. The intensity of the explosion varies with the amount of air present in the ; Durham's Fermentation-tube.—D


. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. 202 BACTERIOLOGY Fig. 37. hydroxide solution gives the proportion of CO2 absorbed. The explosive character of the residue is determined as follows: the thumb is placed again over the mouth of the bulb and the gas from the closed branch is allowed to flow into the bulb and mix with the air there present. The plug is then removed and a lighted match inserted into the mouth of the bulb. The intensity of the explosion varies with the amount of air present in the ; Durham's Fermentation-tube.—Dur- ham employs a convenient modifica- tion of the ordinary fermentation-tube, which is constructed in the following manner: test-tubes of about 10 or-12 capacity are placed in an inverted position within a larger test-tube, and the latter plugged with cotton in the usual way and sterilized. (See Fig. 37.) The small tube should fit loosely within the larger one. The medium to be used is run into the larger tube until there is present about 50 per cent, more than the volume of the smaller tube. The whole is then sterilized in streaming steam by the fractional method. After the first sterilization the small tube will be found almost filled with fluid, over which a small air- bubble lies. After the second or third sterilization this Durham's fermentation- Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Abbott, Alexander Crever, 1860-1935. Philadelphia, Lea & Febiger


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