. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . s especiallyadapted for staining of bacteria in tissues, sputum, etc. It is a positive Grams stain if by application of thismethod either the organism loses none of the stain, orthe is dark blue or dark slate-blue. It is a negativestain if either the coloration is completely gone, or onlya light bluish tinge is left. All stained preparations and all Gram stains must bepreserved for inspection. 3. Transfer from agar-culture to the following media:Glucose-agar. Litmus
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . s especiallyadapted for staining of bacteria in tissues, sputum, etc. It is a positive Grams stain if by application of thismethod either the organism loses none of the stain, orthe is dark blue or dark slate-blue. It is a negativestain if either the coloration is completely gone, or onlya light bluish tinge is left. All stained preparations and all Gram stains must bepreserved for inspection. 3. Transfer from agar-culture to the following media:Glucose-agar. Litmus transfers, excepting gelatin, are to be placedin the thermostat, unless otherwise ordered. 4. An accurate description is to be made of eachindividual culture (see Chap. X), and outUne sketchesespecially of the gelatin and the milk cultures. Thesedescriptions should be made complete after 24 hours, ROUTINE STUDY OF VARIOUS GROUPS 55 and any changes should be noted after 48 hours and after6 days. (See culture charts.) 5. Plates are to be made in agar from a 24-48-hour-. FlG. 26 Thermostat or Incubator old broth-culture of each bacterium, unless otherwisedirected. These plates are to be described once after24-48 hours. (See Chap. X.) Note.—The thermostat or incubator (Fig. ?6) is a boxmade of copper and having double walls, between which water 56 LABORATORY GUIDE IN BACTERIOLOGY circulates. The outer surface is usually covered with asbestosor linoleum, so as to hold the heat. The thermostat is providedwith two doors, the inner one of glass so as to enable the observerto look inside without opening it, the other one covered like thewalls. A thermometer reaches through the upper part, and sev-eral air-holes are provided in it, which permit free circulationof air. The heat is controlled by Bunsen burners with self-regulating devices. The gas supply is shut off automatically, ifit should accidentally become extinguished. For class use largeincubators are constru
Size: 1478px × 1691px
Photo credit: © Reading Room 2020 / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, bookpublisherchicagotheuniversi
