. A text-book of bacteriology. Bacteriology. 84 CL'LTIVATIOX OF ANAEROBIC BACTERIA. test tube for about half an inch, as shown at b, Fig. 53. The space above the cork is then filled with melted sealing wax, which I have found to prevent leakage better than paraffin, which contracts upon cooling. The test tube is inverted while hydrogen is passed through the tube c, and by reason of its levity the gas quickly passes through the cotton air filter and displaces the oxygen in the test tube (Fig. 54). After allowing the gas to flow for a few minutes the outlet tube is first sealed in a flame and th
. A text-book of bacteriology. Bacteriology. 84 CL'LTIVATIOX OF ANAEROBIC BACTERIA. test tube for about half an inch, as shown at b, Fig. 53. The space above the cork is then filled with melted sealing wax, which I have found to prevent leakage better than paraffin, which contracts upon cooling. The test tube is inverted while hydrogen is passed through the tube c, and by reason of its levity the gas quickly passes through the cotton air filter and displaces the oxygen in the test tube (Fig. 54). After allowing the gas to flow for a few minutes the outlet tube is first sealed in a flame and then the inlet tube. As the cotton filter is interposed between the rubber stopper and the culture mate- rial, no special precautions need be taken for the sterilization of the rubber cork and the glass tubes which it carries. h. Fig. 54. Fig. 55. This method is more convenient than that previously described, and the only objection to it is that the oxygen is not completely re- moved from the film of solid gelatin or agar attached to the walls of the test tube. But by passing the hydrogen for a long time it would seem that by diffusion the oxygen remaining in this thin layer would be gotten rid of. At all events, this method will serve for all except the very strict anaerobics. Method of Esmarch.—The following method has been proposed by Esmarch ; Three roll tubes are made in the usual way, and into these liquid gelatin, that is nearly cooled to the point of becoming solid, is poured. This fills the tube without melting the layer of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sternberg, George Miller, 1838-1915. New York, W. Wood and company
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