. Sacbrood. Bees. 32 BULLETIN 431, V. S. DEPARTMENT OP AGEIGTJLTUEE. METHODS USED IN MAKING EXPERIMENTAL INOCULATIONS. The laboratory study of bee diseases being new, it has been neces- sary in many instances to devise new methods. In the experimental inoculations of bees the methods used have undergone revision from time to time. Those now employed have proved quite satis- factory. As the virus of sac- brood has not been cultivated in the lab- oratory artificially, it has been necessaryin these investigations to inoculate a large number of colonies. A nucleus of bees that could be accom- moda
. Sacbrood. Bees. 32 BULLETIN 431, V. S. DEPARTMENT OP AGEIGTJLTUEE. METHODS USED IN MAKING EXPERIMENTAL INOCULATIONS. The laboratory study of bee diseases being new, it has been neces- sary in many instances to devise new methods. In the experimental inoculations of bees the methods used have undergone revision from time to time. Those now employed have proved quite satis- factory. As the virus of sac- brood has not been cultivated in the lab- oratory artificially, it has been necessaryin these investigations to inoculate a large number of colonies. A nucleus of bees that could be accom- modated on from 3 to 6 brood frames was found to serve very satisfactorily the purpose of an ex- perimental colony. The queen should al- ways be clipped. The frames are placed in one side of a 10-frame hive body (fig. 28). Over the entrance to the hive is placed wire cloth, leaving a small space of about 1 inch in length on the side occupied by the brood frames. Petri dishes' (fig. 29) serve well the purpose of a feeder. Both halves of the dish are used as receptacles. These are placed, preferably about four of the halves, within the hive on the bottom board on the side not occu- pied by frames. The hives of the experimental apiary (PL III) are arranged chiefly in pairs, with the entrances of consecutive rows pointing in opposite directions. The space occupied by the apiary should be 1A Petri dish, a much-used piece of apparatus in a laboratory, is simply a shallow, circular, glass dish, with a flat bottom and perpendicular sides. It consists of two halves, a bottom and a top. These are very similar. The top half, being slightly larger, fits over the bottom one when the twohalvesareplacei Fig. 28.—The hive as it is employed to house and feed a colony used for experimental Inoculations. Here are shown four Hoflman frames, a division board, four open Petri dishes as feeders, and the en- trance nearly closed with wire cloth, the opening being on the side of the hive body occupied by
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Keywords: ., bookcentury1900, bookdecade1910, bookpublisherwa, booksubjectbees
