. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHOLINE REQUIREMENT OF MOINA 361 o> CO CO o m. (1009 + 308) (7451249) I .0 mg % (622 + 277) (5971242) (293+132) 8 9 HARVEST DAY 10 FIGURE 1. Effect of increasing dietary egg lecithin (70% pure) levels on the growth of Moina macrocopa fed an artificial diet. Control diet = mg% lecithin. Liver infusion was present, soluble choline (as choline dihydrogen citrate from vitamin mix) = mg%. Points for each day represent average biomass of five observations. (N) = average + SD biomass of al
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHOLINE REQUIREMENT OF MOINA 361 o> CO CO o m. (1009 + 308) (7451249) I .0 mg % (622 + 277) (5971242) (293+132) 8 9 HARVEST DAY 10 FIGURE 1. Effect of increasing dietary egg lecithin (70% pure) levels on the growth of Moina macrocopa fed an artificial diet. Control diet = mg% lecithin. Liver infusion was present, soluble choline (as choline dihydrogen citrate from vitamin mix) = mg%. Points for each day represent average biomass of five observations. (N) = average + SD biomass of all 20 observations for a particular diet. Standard error of the mean for any diet on any day = ng. Average population mortality < 2%, except for populations grown on diet with no lecithin. soluble choline as choline dihydrogen citrate (diets D, E, F, Table II) further enhanced growth rates and reduced mortality. At 2 mg% choline dihydrogen citrate ( mg% choline, diet F) population biomass was not significantly different (P < ) than that of the pure egg lecithin diet (experiment II, Fig. 2) containing mg% choline in the particulate form. The relative amounts of soluble and particulate sources of choline needed to achieve comparable growth rates indicate that a particulate source of choline is 10 times more efficient than a soluble source for these filter feeders. Comparing the composite biomass data of the control diet with diet A, and that of diet C with diet D (Table II), suggests that the choline available from the vitamin mix additive ( mg%) made no significant contribution to the population bio- masses (P < ). Experiments IV and V When Moina was fed diet A (Table III), which lacked both liver infusion and egg lecithin, growth was slow, and almost 40% of the population died. The amount of lecithin in the liver infusion was negligible (G. Holz, Suny, Upstate Medical Center, personal communication), but quantitative analysis showed that
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
