. Diseases of bees. Bees. SACBEOOD. 29 In the study of the virus of sacbrood use has been made also of the Pasteur-Chamberland filter ' (fig. 27). This is a clay filter, the pores of which are much finer than those of the Berkefeld used. In using this filter, an aqueous suspension of larvse dead of the disease is prepared as before. This is filtered by the aid of pressure obtained. Pig. 27.—A convenient apparatus wmch can be employed in using the Pasteur-CIiamberland, Berkefeld, and other filters. Pasteur-Chamberland filter (b) with a glass mantle (a), arubber stopper (c) through which passes
. Diseases of bees. Bees. SACBEOOD. 29 In the study of the virus of sacbrood use has been made also of the Pasteur-Chamberland filter ' (fig. 27). This is a clay filter, the pores of which are much finer than those of the Berkefeld used. In using this filter, an aqueous suspension of larvse dead of the disease is prepared as before. This is filtered by the aid of pressure obtained. Pig. 27.—A convenient apparatus wmch can be employed in using the Pasteur-CIiamberland, Berkefeld, and other filters. Pasteur-Chamberland filter (b) with a glass mantle (a), arubber stopper (c) through which passes the filter, a connecting rubber tubing (d), glass tubing (e), a perforated rubber stopper (f), a vacuum jar (g), designed by the writer, in which is placed a cotton-stoppered and steril- ized flask, a glass stopcock (h), a vacuum gauge (i), a reservoir (m) with pressure-rubber connections 0), and a vacuum pump (k). (Original,) by means of a partial vacuum in an apparatus devised for this pur- pose. Filtrates obtained from this filter when fed to healthy colonies produced the disease. Since the virus of sacbrood wiU pass through iThe Pasteur-Chamberland filter consists of clay molded in the form of a hollow cylinder and baked. This is used with a glass cylinder (a) fitted with a rubber stopper (c). In the use of this filter, force is employed. This was obtained for these experiments through the use of a jar (g) devised by the writer in which a partial vacuum can be produced. In this jar, is placed a flask plugged with cotton and sterilized. Connections are made as shown in the illustration, the vacuum being produced through the use of the pump (k). In less than half an hour usually a half-pint of filtrate can be obtained with this Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original
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