Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . pare them with those shown in Fig. 57, below. (b) Sulphuric Acid Test (Salkowski).—Dissolve a few crystals of cholesterolin a Uttle chloroform and add an equal volxmie of concentrated sulphuric play of colors from bluish-red to cherry-red and purple is noted in the chloro-form while the acid assmnes a marked green fluorescence. (c) Acetic Anhydride-H2S04 Test (Liebermann-Burchard).—Dissolve afew crystals of cholesterol in 2 of chloroform
Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . pare them with those shown in Fig. 57, below. (b) Sulphuric Acid Test (Salkowski).—Dissolve a few crystals of cholesterolin a Uttle chloroform and add an equal volxmie of concentrated sulphuric play of colors from bluish-red to cherry-red and purple is noted in the chloro-form while the acid assmnes a marked green fluorescence. (c) Acetic Anhydride-H2S04 Test (Liebermann-Burchard).—Dissolve afew crystals of cholesterol in 2 of chloroform in a dry test-tube. Now add 10drops of acetic anhydride and 1-3 drops of concentrated sulphuric acid. Thesolution becomes red, then blue, and finally bluish-green in color. This reactionis used in the quantitative determination of cholesterol (see Chapter XVI). (d) Iodine-sulphuric Acid Test.—Place a few crystals of cholesterol in one ofthe depressions of a test-tablet and treat with a drop of concentrated sulphuric acidand a drop of a very dilute solution of iodine. A play of colors consisting of violet,blue, green, and red Fig. 57.—Cholesterol. (e) Schiffs Reaction.—To a little cholesterol in an evaporating dish add a fewdrops of a reagent made by adding i volume of 10 per cent ferric chloride to 3 vol-umes of concentrated sulphuric acid. Evaporate to dryness over a low flame andobserve the reddish-violet residue which changes to a bluish-violet. 9. Preparation of Taurine.—To 300 of bile in a casserole add 100 ofhydrochloric acid and heat until a sticky mass (dyslysin) is formed. This pointmay be determined by drawing out a thread-like portion of the mass by means of aglass rod, and if it solidifies immediately and assumes a brittle character we mayconclude that all the taurocholic and glycoholic acid has been decomposed. Decantthe solution and concentrate it to a small volume on the water-bath. Filter thehot solution to remove sodium chloride
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbiochem, bookyear1916
