. The chemistry and mode of action of plant growth substances; proceedings of a symposium held at Wye College, University of London, July 1955. Plant regulators; Auxin; Growth (Plants). Figure 1. Histograms showing the biological activity of substances extracted ivith different solvents from tomato ovary tissues. The unripe tomato fruits (male sterile mutant of the var. John Baer) were harvested 20 days after hand- pollination and lyophilized. 100 mg {dry weight) of the lyophilized powder were extracted for 3 hours at 0 C with the indicated solvent. The crude extracts were concentrated under r
. The chemistry and mode of action of plant growth substances; proceedings of a symposium held at Wye College, University of London, July 1955. Plant regulators; Auxin; Growth (Plants). Figure 1. Histograms showing the biological activity of substances extracted ivith different solvents from tomato ovary tissues. The unripe tomato fruits (male sterile mutant of the var. John Baer) were harvested 20 days after hand- pollination and lyophilized. 100 mg {dry weight) of the lyophilized powder were extracted for 3 hours at 0 C with the indicated solvent. The crude extracts were concentrated under reduced pressure and chromatographed in KOpropanol+2?>°o ammonia +water (80; 10; 10) {vjv). When the solvent front had travelled 20 cm past the initial spot, the paper strips (Whatman No. 1 paper) were air-dried and cut into twenty 1 cm segments. Each of these paper segments was incubated with 0-5 of solution and ten oat coleoptile sections. The elongation of these sections after about 20 hours is plotted along the orditmte axis of the diagrams. The position of the paper segment on the original chromatogram is plotted in Rf units along the other axis. particular instance, ethyl acetate and especially methanol were better than ether. A second example is taken from another fruit, the bean. Lyophilized bean seeds, dissected out of the pods about 8 days after pollination, were extracted for 5 hours at 0°C in the dark with 10 each of absolute methanol, absolute ethanol, or anhydrous ethyl acetate. The aliquot extracted with ethyl acetate was washed at the end with absolute ethanol and the two extracts combined. The chromatograms were developed in the same solvent. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Wye College; Wain, R. L; Wightman, F. (Frank). London, Butterworths Scientific Publicatio
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Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectgrowthplants
