. Biological structure and function; proceedings. Biochemistry; Cytology. 6o8 V. T. NACHMIAS AND J. M. MARSHALL, JR. EFFECTS OF PH ON BINDING The effects of pH on the binding of ferritin and of methyl ferritin to the cell surface were studied. Because the proteins had an intense orange colour, the results of binding and washing experiments could be followed by direct observation of the living cells. The results were confirmed by electron microscopy. At pH 4, when both proteins were positively charged, both were bound, and both invoked the pinocytosis response. At neutral or slightly acid pH,.
. Biological structure and function; proceedings. Biochemistry; Cytology. 6o8 V. T. NACHMIAS AND J. M. MARSHALL, JR. EFFECTS OF PH ON BINDING The effects of pH on the binding of ferritin and of methyl ferritin to the cell surface were studied. Because the proteins had an intense orange colour, the results of binding and washing experiments could be followed by direct observation of the living cells. The results were confirmed by electron microscopy. At pH 4, when both proteins were positively charged, both were bound, and both invoked the pinocytosis response. At neutral or slightly acid pH,. Fig. 2. Binding of ferritin to surfacL- coat ot amoeba C/kius cJiaos, at pH 4. The binding persists after washing at pH 4 for 5 to 10 min. when its particles carried a net negative charge, ferritin was not bound and did not invoke the pinocytosis response. Methyl ferritin was too insoluble at neutral pH to permit a direct comparison of its binding with that of ferritin, but a clear difference in the reversibility of binding was seen in washing experiments. Once bound to the cell surface at pH 4, neither protein was removed by washing in the cold at pH 4. When the washing was done above pH 5, ferritin was quickly removed, but methyl ferritin was not. Figure 2 is an electron micrograph which shows ferritin bound to the cell at pH 4, and remaining bound after washing at the same pH. Note that. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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