. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 240 ELECTRON TRANSPORT .5 - LIGHT OFF. NAD + FUMARATE LIGHT ON 2 3 4 MINUTES Fig. 3. Photooxidation of TMPD by R. rubnan chromatophores. The experimental conditions were as given for Fig. 1, except that mM TMPD was substituted for DPIPH2. The BChl concentration was mg in the 8 ml reaction system. the system. Illumination in this case resulted in a fast reaction fol- lowed by a secondary slower reaction which was coupled to the photo- reduction of the added fumarate. When the light was turned off, a bi- phasic reactio
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. 240 ELECTRON TRANSPORT .5 - LIGHT OFF. NAD + FUMARATE LIGHT ON 2 3 4 MINUTES Fig. 3. Photooxidation of TMPD by R. rubnan chromatophores. The experimental conditions were as given for Fig. 1, except that mM TMPD was substituted for DPIPH2. The BChl concentration was mg in the 8 ml reaction system. the system. Illumination in this case resulted in a fast reaction fol- lowed by a secondary slower reaction which was coupled to the photo- reduction of the added fumarate. When the light was turned off, a bi- phasic reaction was observed. The initial fast back-reaction can be correlated with reduction of methylene blueby the reduced components in the chromatophore, while the secondary back-reaction is due to the enzymatic reduction of methylene blue by the succinate present in the system. In the case of ferrocytochrome c, a reaction was observed in the absence of added oxygen, but the initial reaction was slow and the extent of the reaction was less than that observed with DPIPH2. In this case also, a coupled photooxidation could be obtained when fumarate was added to the system. These reactions observed are in agreement with the data on photooxidations previously reported (4), but the present experiments show there are two phases for the photooxidation of both these compounds. The response of ferrocyanide in the R. riibrum chromatophore system was examined, and the results of this experiment are shown in Fig. 5. Although some absorbancy change was noted when the system was illuminated, this was largely due to absorbancy changes which take. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Symposium on Bacterial Photosynthesis (1963 : Yellow Springs, Ohio); Charles F. Kettering Research Laboratory (Yellow Springs, Ohio); Gest, H
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