Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . he urine. Experiments. 1. Solubility.—Test the solubility of benzoic acid in water,alcohol and ether. 2. Crystalline Form.—Recrystallize some benzoic acid fromhot water, examine the crystals under the microscope and com-pare them with those reproduced in Fig. 94, page 264. 3. Sublimation.—Place a little benzoic acid in a test-tubeand heat over a flame. Note the odor which is evolved andobserve that the acid sublimes in the form of needles. 4. Disso


Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . he urine. Experiments. 1. Solubility.—Test the solubility of benzoic acid in water,alcohol and ether. 2. Crystalline Form.—Recrystallize some benzoic acid fromhot water, examine the crystals under the microscope and com-pare them with those reproduced in Fig. 94, page 264. 3. Sublimation.—Place a little benzoic acid in a test-tubeand heat over a flame. Note the odor which is evolved andobserve that the acid sublimes in the form of needles. 4. Dissolve a little sodium benzoate in water and add a solu-tion of neutral ferric chloride. Note the production of abrownish-yellow precipitate (Salicylic acid gives a reddish-violet color under the same conditions). Add ammonium hy-droxide to some of the precipitate. It dissolves and ferric hy-droxide is formed. Add a little hydrochloric acid to anotherportion of the original precipitate and stand the vessel awayover night. What do you observe? 1 Zeitschrift fur physiologische Chemie, 1897, xxiii, p. 92. 264 PHYSIOLOGICAL CHEMISTRY,Fig. Benzoic Acid. NUCLEO-PROTEID. The nubecula of normal urine has been shown by one investi-gator to consist of a mucoid containing per cent of nitro-gen and per cent of sulphur. This body evidently origi-nates in the urinary passages. It is probably slightly solublein the urine. Some investigators believe that the body form-ing the nubecula of normal urine is nucleo-proteid and not amucin or mucoid as stated above. A discussion of nucleo-proteid and related bodies occurring in the urine under patho-logical conditions will be found on page 296. NH-COI OXALURIC ACID, CO NH2 COOH. Oxaluric acid is not a constant constituent of normal humanurine, and when found occurs only in traces as the ammoniumsalt. Upon boiling oxaluric acid it splits into oxalic acid andurea. URINE. 265 ENZYMES. Various types of enzymes have been isolated from the


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