Archive image from page 34 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 Restriction Endonuclease Digestion and Agarose Gel Electrophoresis P Figure 6. Flowchart of steps required to produce a physical map of a chromosome. This a a very brief overview. Refer to entirety of section for a more complete description of the overall procedures followed. 1. Polymerase Chain Reaction. In order to map the human genome we need techniques that would show that a group of recombinant DNAs contained an STS marker sequenc
Archive image from page 34 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 Restriction Endonuclease Digestion and Agarose Gel Electrophoresis P Figure 6. Flowchart of steps required to produce a physical map of a chromosome. This a a very brief overview. Refer to entirety of section for a more complete description of the overall procedures followed. 1. Polymerase Chain Reaction. In order to map the human genome we need techniques that would show that a group of recombinant DNAs contained an STS marker sequence within its own DNA base sequence. Two broad techniques were explored in this project, polymerase chain reaction and hybridization. The first method of mapping used here mvolves the polymerase chain reaction. The polymerase chain reaction (PCR) is used to amplify a section of DNA found between two primers for a known sequence. There are many of these known sequences, called Sequence-Tagged Site (STS) markers, along the chromosomes. Oligonucleotide primers flank the regions of STS markers and are complementary to the DNA. Taq DNA polymerase, a heat-stable enzyme, is used to catalyze the amplification reaction by replicating the region of DNA between the STS primers. The template DNA strand is 16
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