. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. . \ 'mure 2. Histochcmical properties of 'tin- bender muscle. Semi xec- lions stained for NADU diaphorasc activity (A), myofihrillar A I Paxc activity with alkilme (Bl and acid (C) preincuhation, and for polysac- charide content (D). a: anterior: d: dorsal: p: posterior: \: ventral. Cali- bration: 500^m (A-C)and 415 ^m 11)i length ( ^m: range 8 to 16 /jin). The distal-dorsal quadrant contained a similar population of long-sarco- mere fibers but also had some fibers with shorter (4 to 5 Mm) sarcomeres. Fibers located on t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. . \ 'mure 2. Histochcmical properties of 'tin- bender muscle. Semi xec- lions stained for NADU diaphorasc activity (A), myofihrillar A I Paxc activity with alkilme (Bl and acid (C) preincuhation, and for polysac- charide content (D). a: anterior: d: dorsal: p: posterior: \: ventral. Cali- bration: 500^m (A-C)and 415 ^m 11)i length ( ^m: range 8 to 16 /jin). The distal-dorsal quadrant contained a similar population of long-sarco- mere fibers but also had some fibers with shorter (4 to 5 Mm) sarcomeres. Fibers located on the ventral surface of the bender muscle had sarcomeres between 4 and 16 ^m; the distal-ventral quadrant had a higher proportion of shorter-sarcomere (5 to 8 pm) fibers (Fig. 1). Sections of the dissected carpopoditc revealed that the bender muscle extends along the entire anterior surface and along some of the posterior surface of the segment (Fig. 2). The smaller stretcher muscle (which was re- moved prior to sectioning) is located along the posterior surface of the carpi ipodite and is confined to the ventral and central portion ol'the segment. Bender muscle fibers can he differentiated In their contrasting histochcmical properties. I his can be seen in Figure 2. which shows four serial sections ol'the bender muscle taken at a level about one-third from the proxi- mal end of the carpopodite. There is a population of muscle fibers that displays high NADH diaphorase activ- ity, as seen by dark rings around the margin of individual fibers (Fig. 2A). In all preparations (n = 16). these types of fibers were located in the dorsal half of the muscle, on either side of the apodeme. With regard to myofibrillar ATPase activity, these same libers stained poorly with alkaline preincubation (Fig. 2B) but well with acidic pre- incubation (Fig. 2C). Finally, these fibers had a high polysaccharide content (Fig. 2D). Most of the other fibers in the bender muscle showed poor NADH diaphorase ac
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
