. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 296 ARTHUR D. HASLER between pH 4-6 which indicated the presence of a katheptic or auto- lytic enzyme of the tissues. The alcoholic extracts of Calanus made by Bond (1934) were most active at pH The secondary opti- mum of his extracts at pH were unconfirmed in this work. The secondary optimum re>ulting from the autolytic enzymes was pi I The discrepancy f pi I optima may be due to variations in extracting technique, for Mansour-Bek (1932) found that crude proteinase extract of Maja squinado had a pH
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 296 ARTHUR D. HASLER between pH 4-6 which indicated the presence of a katheptic or auto- lytic enzyme of the tissues. The alcoholic extracts of Calanus made by Bond (1934) were most active at pH The secondary opti- mum of his extracts at pH were unconfirmed in this work. The secondary optimum re>ulting from the autolytic enzymes was pi I The discrepancy f pi I optima may be due to variations in extracting technique, for Mansour-Bek (1932) found that crude proteinase extract of Maja squinado had a pH optimum on gelatin of ; the optimum for purified en/vim- was pH The pH of the buffered substrates used in this experiment was determined with the aid of the glass electrode. Am\lasc.—Both Diaptomus and Calanus extracts contained active amylase which hydrolyzed starch. The iodometric titration previously. IMC. 7. 11 ydntlyMs f trilmtyrin by lipases of I)iti/^!/>iinis (/') and Calantts (C). The nnlinak' indicate the miniluT uf cc. of N/20 NaOH ; the abscissa is the time in hours. describee] was used as a criterion of starch hydrolysis. The amounts of N/40 Xa.,S,().. present in 10 cc. of digest can be read from Fig. 6. These figures are equivalent to the amount of N/40 reducing sugars lib- erated by hydroKsis. In both instances 5 cc. of 1 per cent glycerol extract were added to 25 cc. of 3 per cent starch at pll and 25 cc. of water and incubated at 37° C. At intervals 10-cc. samples were with- drawn and the reducing groups titrated. Lipasc.—Trilmtyrin was attacked by an esterase present in both Di- aptomus and Calanus extracts. Fifty cc. of 4 per cent tributyrin were emulsified with sodium glycocholate to which were added 5 cc. of 1 per cent extract of Diaptomus; extracts of Calanus were examined by the same procedure. At intervals 10-cc. aliquots were withdrawn and ti- trated with X, 20 XaOII. The results are shown in Fig. Please note that these image
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
