. The Biological bulletin. Biology; Zoology; Marine biology. /3-GALACTOSIDASE IN DROSOPHILA 181 Larval spiracle. M^ Adult air sacs Figure 7. Schematic summary through metamorphosis of endogenous /J-galactosidase activity stained at pH Based on X-gal-stained 20-Mm serial frozen longitudinal sections of pupal stages 1-15 cut in the frontal plane. At stage P4. staining (black) is primarily localized to the region housing the imaginal discs, remodeling midgut, and cellular epidermis. After head eversion, P5, there is light staining of the head, thoracic and abdominal fat-body tissue, with dar
. The Biological bulletin. Biology; Zoology; Marine biology. /3-GALACTOSIDASE IN DROSOPHILA 181 Larval spiracle. M^ Adult air sacs Figure 7. Schematic summary through metamorphosis of endogenous /J-galactosidase activity stained at pH Based on X-gal-stained 20-Mm serial frozen longitudinal sections of pupal stages 1-15 cut in the frontal plane. At stage P4. staining (black) is primarily localized to the region housing the imaginal discs, remodeling midgut, and cellular epidermis. After head eversion, P5, there is light staining of the head, thoracic and abdominal fat-body tissue, with darker staining associated with the pupal spiracle. Developing legs and wings exhibit light diifuse staining of foamy-appearing tissue characteristic of the imaginal tissues. In stages P7 and P8. punctate staining intensifies in thoracic and abdominal fat body. Dark staining appears in the first dorsal anastomosis between the two spiracles. Lighter staining persists in developing legs and wings. A concentrated "ball" of stain is seen at the tip of the abdomen. Staining decreases dramatically at stage P9. In stages P10-P14, dark staining is only associated with the pupal spiracle region and in a region where the meconium is found. The fat body and developing digestive system show low levels of staining. (Anatomy after Bainbridge and Bownes, 1981; tracheal anatomy after Whitten, 1980.) of Stain appeared at the tip of the abdomen. As with the earlier stages, there was little or no staining of compact tissues. The staining associated with the developing ap- pendages, pupal spiracle, and trachea persisted. In stage P9, the stain in the thoracic and abdominal fat body decreased and remained at low levels through PI4. At stage PIO and in subsequent pupal stages, no stain was found in the developing appendages. Through stages P9-P14, intense stain was associated with the anterior spiracle region, along the line of the first dorsal anastomosis and into the dorsal tracheal trunks
Size: 2081px × 1201px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1800, bookdecade1890, booksubjectb, booksubjectzoology
