. Biological structure and function; proceedings. Biochemistry; Cytology. PHOTOSYNTHETIC PHOSPHORYLATION AND THE ENERGY CONVERSION'PROCESS 375 TABLE VII Photoproduction of Hydrogen from Succinate by Chromatium (Ogata, Nozaki, and Arnon [91]) Treatment 1. Complete 2. Complete, KOH omitted 3. Complete, dark 4. Complete, succinate omitted 1. Complete 2. Complete, succinate omitted 3. Complete, plus palladium asbestos 4. Complete, plus carbon monoxide ftmoles H; evolved 8-6 The complete system contained, in a final volume of 3-0 ml., 100 mg. wet cells that were suspended in a modified nutrient sol
. Biological structure and function; proceedings. Biochemistry; Cytology. PHOTOSYNTHETIC PHOSPHORYLATION AND THE ENERGY CONVERSION'PROCESS 375 TABLE VII Photoproduction of Hydrogen from Succinate by Chromatium (Ogata, Nozaki, and Arnon [91]) Treatment 1. Complete 2. Complete, KOH omitted 3. Complete, dark 4. Complete, succinate omitted 1. Complete 2. Complete, succinate omitted 3. Complete, plus palladium asbestos 4. Complete, plus carbon monoxide ftmoles H; evolved 8-6 The complete system contained, in a final volume of 3-0 ml., 100 mg. wet cells that were suspended in a modified nutrient solution with nitrogen omitted, and the following in micromoles; tris buffer, pH 7-2, 80; AlgCl,, 5 ; succinate, 20. o- I ml. of 20",, KOH was present in the centre well. The reaction was carried out for 2 hr. at 30 in argon. Illumination 35 000 Lux. thiosulphate was also inhibited by molecular nitrogen and ammonium ions. The results are shown in Fig. 19. The photoproduction of hydrogen was dependent on the concentration of thiosulphate and was abolished by heating the cells (Fig. 20). Growing Chromatium cells were found, by analysis to have oxidized in 4 days 27 millimoles of added thiosulphate into 54 millimoles of sulphate. During. 30 60 90 120 150 minutes Fig. 17. Photoproduction of hydrogen gas from succinate by Chromatium cells. Experimental conditions as described in Table \ll (Ogata, Xozaki, and Arnon [91]).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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