. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. -14 -10 -6 log Glycine Cone., M -10 log Glycine Cone., M. -14 -10 -6 log Glycine Cone., M 14 -10 -6 log Glycine Cone., M Figure 3. Afferent and efferent cnida dose-responses to glycine. Combined responses from Type B plus Type C cnidocyte/supporting cell complexes (CSCCs) are measured in seawater (open circles). Cnida responses from Type Cs only are measured in the presence of 1CTS M D-600 in seawater (closed circles). Calculated responses from Type Bs (closed squares) are obtained by subtracting Type C responses from combin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. -14 -10 -6 log Glycine Cone., M -10 log Glycine Cone., M. -14 -10 -6 log Glycine Cone., M 14 -10 -6 log Glycine Cone., M Figure 3. Afferent and efferent cnida dose-responses to glycine. Combined responses from Type B plus Type C cnidocyte/supporting cell complexes (CSCCs) are measured in seawater (open circles). Cnida responses from Type Cs only are measured in the presence of 1CTS M D-600 in seawater (closed circles). Calculated responses from Type Bs (closed squares) are obtained by subtracting Type C responses from combined Type B plus Type C responses. Values express the mean of duplicate experiments. Each experi- ment consists of replicate probes for each glycine concentration; each probe and each tentacle is used only once. Vertical bars represent standard error of the mean and are applied to all measured means, but not to calculated means. (A) Effect of glycine on nematocyst discharge (n = 15). (B) Effect of glycine on spirocyst discharge (n = 15). (C) Effect of glycine on nematocyst-mediated adhesive force (n = 14). (D) Effect of glycine on calculated intrinsic adherence. downward trend in imL with increasing glycine concentra- tions. Glycine suppresses /,„''. In contrast to NANA. in which NANA-sensitized discharge of nematocysts from Type Bs spans a narrow range of NANA concentrations (Fig. 2A. closed squares), glycine-sensitized discharge of nemato- cysts spans a wide range of glycine concentrations (Fig. 3A, closed squares). At all tested glycine concentrations (except 1CT"1 M), the numbers of nematocysts discharged from Type Bs exceed those discharged from Type Cs. Yet the values of imh in glycine never exceed mgf/nemato- cyst ( /jN/nematocyst; Fig. 3D, closed squares), unlike the values in NANA which never drop below mgf/ nematocyst ( ^/N/nematocyst; Fig. 2D, closed squares). Discussion Cnidarians capture swimming prey by discharging nematocysts from their tenta
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