. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. erum-free culture media ordinar-ily employed for the cultivation of bacteria—viz., on nutrient agarand gelatin, in bouillon. The best medium for its growth is a mixtureof one-third human or animal blood serum or ascitic or pleuritic THE DIPLOCOCCUS OF PNEUMONIA. 383 fluid and two-thirds bouillon, or nutrient agar streaked with human orrabbit blood. Growth on Agar.—Cultivated on plain nutrient agar, after twenty-four to forty-eight hours at 37° C, the deep colonies are
. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. erum-free culture media ordinar-ily employed for the cultivation of bacteria—viz., on nutrient agarand gelatin, in bouillon. The best medium for its growth is a mixtureof one-third human or animal blood serum or ascitic or pleuritic THE DIPLOCOCCUS OF PNEUMONIA. 383 fluid and two-thirds bouillon, or nutrient agar streaked with human orrabbit blood. Growth on Agar.—Cultivated on plain nutrient agar, after twenty-four to forty-eight hours at 37° C, the deep colonies are hardly visibleto the eye. Under the microscope they appear light yellow or brownin color and finely granular. The surface colonies are large, equallingin size those of streptococci, but are usually more transparent. Ifblood serum or ascitic fluid be added to the agar the individual coloniesare larger and closer together, and the growth is more distinct in con-sequence and of a grayish color. The surface colonies are almost circu-lar in shape under a magnification of 60 diameters, finely granular in Fig. 124 Fic. 125.
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