. Pathological technique; a practical manual for workers in pathological histology and bacteriology. rge numbers ofthe bacilli. The organism multiplies post-mortem in theblood of the animal and produces the gas. This effectupon the tissues of the dead animal is characteristic of thebacillus. The subcutaneous inoculation of guinea-pigs with youngcultures may produce fatal gas phlegmons. The hemor-rhagic fluid from the dead animal isvirulent for other guinea-pigs, and maybe virulent for rabbits. Gas-production is marked in agar-agarand gelatin cultures containing gas produced burns w
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. rge numbers ofthe bacilli. The organism multiplies post-mortem in theblood of the animal and produces the gas. This effectupon the tissues of the dead animal is characteristic of thebacillus. The subcutaneous inoculation of guinea-pigs with youngcultures may produce fatal gas phlegmons. The hemor-rhagic fluid from the dead animal isvirulent for other guinea-pigs, and maybe virulent for rabbits. Gas-production is marked in agar-agarand gelatin cultures containing gas produced burns with a blueflame and is odorless. Gelatin is liquefied slowly and to alimited extent. ^ ^^ZfZ Glucose Bouillon.—Diffusely clouded glass preparation from theat first, later becoming clearer, with an spleen, stained by whitish, more or less viscid H Sm^s ™eth°d;. * 1500 (W. H. Smith; sediment. photo, by L. S. Brown). Milk.—Coagulated, the clot beingfirm, retracted, and furrowed with the marks of gas-bubbles. Potato.—Growth thin, moist, and grayish-white, or it maynot be 33^ PATH OLD GICA L TE CHNIQ UE. The vitality of the organism depends upon the characterof the culture-medium and the mode of cultivation. It sur-vives longer when cultivated by Buchners method (seepage 223) than when cultivated under hydrogen. Cultureson glucose media are shorter lived than those on plain media. Occurrence.—Occurs at autopsies in which gas-bubbles arepresent in the larger vessels, accompanied by the formationof numerous small cavities in the liver containing gas. Ithas been found also in shell wounds, emphysematousphlegmons, in puerperal sepsis, in peritonitis, and in otherconditions. It is a normal inhabitant of cultivated soil andof feces. Bacillus of Tetanus.—This bacillus will not grow inthe presence of oxygen.
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