. Biophysical science. Biophysics. 17:4/ Enzyme Kinetics of Hydrolytic Reactions 327 This equation also predicts a straight line; its graph is also called a Lineweaver-Burk plot. It is illustrated in Figure 7b, where xJV is plotted against x. It is seen that the best points, obtained at large x, are now spread out over a major part of the graph instead of being cramped near the axis. The strongest point of the foregoing Michaelis-Menten formulation is that it is the simplest theory which can be fitted to the reactions of most hydrolases. It also fits the reactions of several other types of enz
. Biophysical science. Biophysics. 17:4/ Enzyme Kinetics of Hydrolytic Reactions 327 This equation also predicts a straight line; its graph is also called a Lineweaver-Burk plot. It is illustrated in Figure 7b, where xJV is plotted against x. It is seen that the best points, obtained at large x, are now spread out over a major part of the graph instead of being cramped near the axis. The strongest point of the foregoing Michaelis-Menten formulation is that it is the simplest theory which can be fitted to the reactions of most hydrolases. It also fits the reactions of several other types of enzymes. Its weakest point is that the intermediate complex has not been directly 1/1/. x/v Slope = \/Vmax K^/Vmox (b) Figure 7. (a) Lineweaver-Burk plot of the hydrolysis of sucrose by sucrase. (b) Modified Lineweaver-Burk plot. After F. M. Huennekens, "Biological Reactions: Measurement and General Theory," Technique of Organic Chemistry, Vol. 8, Investigations of Rates and Mechanisms of Reactions, S. L. Friess and A. Weissberger, eds. (New York: Interscience Publishers, Inc., 1953). observed for most reactions; it could easily be an oversimplification. In spite of these uncertainties, this type of kinetics is the basis for many studies. Almost all enzyme kinetics are described in the language of intermediate complexes and Michaelis constants. 4. Action of Inhibitors Many enzyme reactions have been studied in part through the use of inhibitors. Specific inhibitors are useful for determining the role of particular enzymes. Other inhibitors (such as para-chloro-mercuri- benzoate, PCMB) are useful in determining the activity of certain groups (for example, sulfhydryl groups) in the enzyme activity. In this section, the action of inhibitors for systems obeying Michaelis-Menten kinetics will be Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustration
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