Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . rmeier. 2 ^ Noguchi (Jour. Exper. Med., 1912, XVI, 199) has succeeded in cultivating this organismand shows that this pathogenicity is not thus diminished. Nicolle, Blaizot and Conseil (Ann. de IInst. Pasteur, 1913, XXVII, 204) show that thebite of the louse does not transmit this disease as is commonly supposed. If the louse becrushed on the skin and there are abrasions on the skin from scratching, the parasites gainaccess to the system. THE BLOOD. 575 (3). Sleeping Sickness. This ver


Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . rmeier. 2 ^ Noguchi (Jour. Exper. Med., 1912, XVI, 199) has succeeded in cultivating this organismand shows that this pathogenicity is not thus diminished. Nicolle, Blaizot and Conseil (Ann. de IInst. Pasteur, 1913, XXVII, 204) show that thebite of the louse does not transmit this disease as is commonly supposed. If the louse becrushed on the skin and there are abrasions on the skin from scratching, the parasites gainaccess to the system. THE BLOOD. 575 (3). Sleeping Sickness. This very interesting condition which is so prevalent in Central andWest Africa seems to be due to an actively motile fusiform flagellate knownas the trypanosoma Gambiense, which can be found in the blood free in theplasma (never intracorpuscularly), moving with a screw-like motion amongthe red cells which it does not seem to disturb. This parasite doubtless hasa sexual development, its host being the common fly, Glossina palpalis, whileclosely related trypanosomata are transmitted by the bites of various flies,. Fig. 146.—Trypanosoma gambiense. {Da Costa.) especially one of the seven varieties of the tsetse fly. This organism is fromtwo to three times as long as a red blood-corpuscle (18 to 25 microns) and 2 to21/2 microns wide, having a flagellum anteriorly and an undulating membraneextending its entire length.^ In the fresh blood specimen these parasitesshould be looked for with only a medium magnification. These parasitesvary much in number sometimes being absent for a long period and then suddenlyreappearing in large numbers. Symptoms of the disease seem to bearlittle relation to the number of parasites in the peripheral blood, so that insome cases it may be necessary to examine the fluid in the edematous areasor even to puncture the cervical When these parasites arestained with a polychrome dye they show a rather large red nucleus about themiddle, a centrosome st


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