. The actinomycetes. Actinomycetales. THERMOPHILIC 305 Aerial mycelium chalk-white. Cultures are thermophilic, facultatively aerobic. Aerial spores produced in 3 to 4 days in hanging drops. Spores formed on simple or branched sporophores. Spores oval, later round. Sucrose nitrate agar: Growth colorless. Aerial mycelium moderate. Glycerol-asparagine agar: Growth abun- dant, yellow. Aerial mycelium produced. Nutrient agar: Growth good, yellow. Aerial mycelium thick. Potato: Growth yellow, partly covered with aerial mycelium. Soluble pigment light brown. Starch: No hydrolysis. Nitr
. The actinomycetes. Actinomycetales. THERMOPHILIC 305 Aerial mycelium chalk-white. Cultures are thermophilic, facultatively aerobic. Aerial spores produced in 3 to 4 days in hanging drops. Spores formed on simple or branched sporophores. Spores oval, later round. Sucrose nitrate agar: Growth colorless. Aerial mycelium moderate. Glycerol-asparagine agar: Growth abun- dant, yellow. Aerial mycelium produced. Nutrient agar: Growth good, yellow. Aerial mycelium thick. Potato: Growth yellow, partly covered with aerial mycelium. Soluble pigment light brown. Starch: No hydrolysis. Nitrate reduction: Weak. Milk: Unchanged in 16 days. Gelatin: Not liquefied. Temperature: Optimum growth at .">()('. Limited growth at 28 and 65°C. Habitat: Fresh cow manure and manure compost. 2. Thermomonospora fusca (Waksman et ill., 1939) Henssen (Henssen, A. Arch. Mikrobiol. 26: 373-414, 1957). Morphology: Aerial hyphae 20 to 30 /j, long. Spores round to Since the Spores are not produced on the substrate mycelium as in Micromonospora, but ex- clusively on the aerial mycelium, the species was transferred from Micromonospora fusca to Thermomonospora fusca. The branching of the substrate mycelium is monopodial. The hyphae are long, straight, and form straight side branches. The branching is so characteristic, as compared to all the other thermophilic species, that this species can easily l>e recognized. The aerial mycelium is colored brown (Fig. 60). Sucrose nitrate agar: Growth at 28°C deep gray; at 50°C, growth is dark brown to violet. Aerial mycelium gray to lavender. Soluble pigment brown. (relatin: Liquefied. Potato: Growth brown-colored. No aerial mycelium. Soluble pigment ] [Guee (>(). Thermo monosport fusca i Etepro- due id from: Henssen, A. Arch Mi km biol. 2: 401 L957). Milk: No coagulation; slight peptoniza- tion. Habitat: Horse manure. Starch: Hydrolysis. Nitrate reduction: Slight. Cellulose: Growth good. Temperature: Growth and ae
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