. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 284 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 1. (Continued) unfertilized eggs and early embryos of all stages as well as pro- tein of similar size in human HeLa cell extracts (Fig. Ib). This protein is thought to represent the urchin homolog of the cata- lytic subunit of DNA-PK. Lastly, we used the anti-human p460 monoclonal serum to immunostain Arhacia eggs and embryos (Fig. Ic). The urchin p460-related protein appears to be exclusively cytoplasmic in unfertilized eggs and then undergoes a change in localiza
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 284 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 1. (Continued) unfertilized eggs and early embryos of all stages as well as pro- tein of similar size in human HeLa cell extracts (Fig. Ib). This protein is thought to represent the urchin homolog of the cata- lytic subunit of DNA-PK. Lastly, we used the anti-human p460 monoclonal serum to immunostain Arhacia eggs and embryos (Fig. Ic). The urchin p460-related protein appears to be exclusively cytoplasmic in unfertilized eggs and then undergoes a change in localization to the nuclei in cleaving embryos. These results confirm the presence of a DNA-dependent pro- tein kinase activity in fertilized urchin eggs. In vitro kinase as- says reveal that the localization of this activity changes from the cytoplasm in fertilized eggs to the nuclei in later stages of development. A protein related to human p460 is present in all stages o( Arhacia development and may represent the catalytic subunit of urchin DNA-PK. The change in localization of this p460-related protein from the cytoplasm to the nuclei after fer- tilization is consistent with the change in localization of DNA- inducible kinase activity in specific in vitro kinase assays. Al- though the role of the DNA-PK activity in Arhacia develop- ment is not yet known, we suggest that the enzyme is activated upon fertilization and may be required during the early rapid cell cycles in embryogenesis. Supported by a grant from N1H (AR 32549) and an award from the Mason Trust. was supported by Miller and Associates Fellowship (1996) from the MBL. We thank Drs. T. Carter and W. Dynan for providing the antibodies and helpful discussions. Literature Cited 1 Carter, T., I. \ancuro\a, I. Sun, W. Lou, and S. DeLeon. 1990. Mul Cell lltol 1(1:6460-6471. 2. Lees-Miller, S. P., V. Chen, and C. W. Anderson. 1990. M»l Cell Bin/ 10:6472-6481. 3. Gottlieb,!. M., and 1993. CW/72: I 31-142. 4. Har
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