. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOCYAXIN IX HALIOTIS 465 Hemocyanin concentration Figure 4 shows the relationship between the ubsorbance at 560 niju and the copper concentration of the plasma from H. jidgcns, H. corrmjata and //. rnfesccns. Jt is clear that, for the first two species named above, the absorbance at 560 m/x. is well correlated with the copper concentration. This indicates that most of the copper is present in combination as hemocyanin. For both species the y-intercept 600 500 o 2400 o300 o <r \- < O [- 200 100. y = + 7 37 y
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOCYAXIN IX HALIOTIS 465 Hemocyanin concentration Figure 4 shows the relationship between the ubsorbance at 560 niju and the copper concentration of the plasma from H. jidgcns, H. corrmjata and //. rnfesccns. Jt is clear that, for the first two species named above, the absorbance at 560 m/x. is well correlated with the copper concentration. This indicates that most of the copper is present in combination as hemocyanin. For both species the y-intercept 600 500 o 2400 o300 o <r \- < O [- 200 100. y = + 7 37 y = + 10 20 30 COPP ER, mg/ L 40 FIGURE 3. Total organic nitrogen concentration in plasma from 4 species of Haliotis rs. the total copper concentration. The plotted points for each succeeding species have been displaced upward by 100 units. is above 0, indicating that the plasmas absorbed or scattered light by an amount greater than could be attributed to the hemocyanin alone. From the relationships that have been developed it seems that one can with some confidence use the total nitrogen, the absorbance at 560 m/t or at 280 m/A, or the copper concentration, to estimate the hemocyanin concentration in the plasma of all species investigated except //. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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