. Colchicine in agriculture, medicine, biology, and chemistry. Colchicine; Colchicine. Experimental Growth in Animals 215 jected with "theelin" alone showed 20 to 30 dividing cells. After colchicine, this was increased to more than 1500 in about 10 hours. In one experiment in which "theelin" and colchicine were injected simultaneously, the authors wrote that "the general impression is that approximately every other cell is in ;* I'hese results aroused great interest, and marked one of the starting points for. Fig. —Graphical representation of the course


. Colchicine in agriculture, medicine, biology, and chemistry. Colchicine; Colchicine. Experimental Growth in Animals 215 jected with "theelin" alone showed 20 to 30 dividing cells. After colchicine, this was increased to more than 1500 in about 10 hours. In one experiment in which "theelin" and colchicine were injected simultaneously, the authors wrote that "the general impression is that approximately every other cell is in ;* I'hese results aroused great interest, and marked one of the starting points for. Fig. —Graphical representation of the course of cell division in a fibroblast culture treated by colchicine (1/20,000,000). During the two first hours, no notable changes. Later, progressive accumulation of arrested mitoses. Each horizontal line represents one mitosis; it is interrupted at the end of metaphase. Any vertical line indicates th- number of visible mitoses at one moment, that is to say, the mitoses which should b seen in a fixed preparation. This number progressively increases under the influenc of colchicine. The rate of apparition of new prophases is not disturbed with this con- centration. There is no mitotic stimulation. (From a cine-micrographic recording. After Bucher, 1939) e le ce colchicine research outside of the Brussels laboratory. Together with the discovery of colchicine polyjjloidy in 1937, this study initiated the publication of a great number of papers in which colchicine was mainly considered as a tool for making mitotic growth more visible and easier to analyze. However, any tool has its advantages and its shortcomings. Many workers do not appear to have considered carefully the fundamental problems involved in what Allen called the "freezing" of mitoses. * p. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the orig


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