. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SURFACE ANTIGENS IN REAGGREGATION 141 ferred to sea water, it is readily apparent that the cells remained alive in the versenate solution. The above experiments offer additional evidence to support the conclusion that calcium deficiency prevents reaggregation by the inhibition of ameboid movement and does not affect or alter the adhesiveness of the cells to each other. Effects of calcium excess on reaggregation. Although no role of calcium in cell adhesion is indicated by the effects of calcium deficiency, one might be detec
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SURFACE ANTIGENS IN REAGGREGATION 141 ferred to sea water, it is readily apparent that the cells remained alive in the versenate solution. The above experiments offer additional evidence to support the conclusion that calcium deficiency prevents reaggregation by the inhibition of ameboid movement and does not affect or alter the adhesiveness of the cells to each other. Effects of calcium excess on reaggregation. Although no role of calcium in cell adhesion is indicated by the effects of calcium deficiency, one might be detected by the effects of calcium excess. Therefore, one gram of Microciona was dissociated into 40 ml. of normal sea water and two ml. of the suspension were added to eight ml. of normal sea water, and to seven ml. normal sea water plus one ml. of M CaCl2 in Syracuse dishes. 15 14- 13- 12- UJ z 10- K UJ °- 9- in UJ 5 7- (9 < K III 4H 2- I- CALCIUM CHLORIDE SEA WATER. 12 15 18 TIME IN HOURS 21 24 27 30 FIGURE 5. Reaggregation of Microciona in normal and high calcium media. Aggregate counts were made at varying intervals; the reaggregation curves are shown in Figure 5. Differences are well within the limits of variation among preparations in sea water. In experiments under identical conditions, Galtsoff (1925) found times as many aggregates (meaning less reaggregation) in excess-calcium solution as in normal sea water at 24 hours. The difference in results is difficult to explain; it leaves considerable doubt whether reaggregation is significantly affected by in- creasing the calcium of the medium times. DISCUSSION The present results show that reaggregation is inhibited by the presence of homologous antibodies. The usual expectation, when a specific antiserum is added to a suspension of the homologous cells, is that an agglutination of the cells will. Please note that these images are extracted from scanned page images that may have been digitally enhanced
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
