. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. METACERCARIA HEMOGLOBIN 177 â u a M o in .0 <. Elution volume (mis) Figure 4. Sephacryl-400 chromatography of metacercaria whole animal lysate. Key to standards: BDN, blue dextran; HMW, Amphitrite HMW vascular hemoglobin; FER. ferritin; MYO. myoglobin. nificant amounts of tritiated glycine in eluted fractions containing proteins which include hemoglobins. Discussion The absorption spectra of the pigment in metacercaria tissues identifies it as a heme protein. The pigment ap- pears to be in all body cells but may also be l
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. METACERCARIA HEMOGLOBIN 177 â u a M o in .0 <. Elution volume (mis) Figure 4. Sephacryl-400 chromatography of metacercaria whole animal lysate. Key to standards: BDN, blue dextran; HMW, Amphitrite HMW vascular hemoglobin; FER. ferritin; MYO. myoglobin. nificant amounts of tritiated glycine in eluted fractions containing proteins which include hemoglobins. Discussion The absorption spectra of the pigment in metacercaria tissues identifies it as a heme protein. The pigment ap- pears to be in all body cells but may also be located in intercellular spaces as in some entosymbiotic rhabdocoel flatworms (Jennings and Cannon, 1985). The metacer- caria hemoglobin exhibited reversible spectral shifts when alternately exposed to oxygen and nitrogen, indi- cating a functional ability to bind oxygen. The absorp- tion maxima for oxy, deoxy and carboxy forms of the hemoglobin closely resemble other functional hemo- proteins, including both host hemoglobins (Weber et al, 1977). Cain (1969a) demonstrated the presence of heme protein in several larval trematode stages by positive ben- zidine reaction. However, this is the first larval trema- tode shown to possess a hemoglobin capable of func- tional oxygen transport. Hemoglobin-containing adult trematode, monogen- ean, nematode, and arthropod parasites are known from many vertebrate and invertebrate hosts (Lee and Smith, 1965; Thurston, 1970; Von Brand, 1973). Many of these parasites live in regions in the host that have low P02 such as the liver or the lumen of the gut. Some, like the nematode Syngamus trachea (Rose and Kaplan, 1972), live in high P02 environments. Hemoglobin-containing turbellarian worms of the families Pterastericolidae and Umagillidae have been identified living in the guts of their echinoderm hosts (Jennings and Cannon, 1985, 1987). As in other animals, hemoglobin in parasitic or endocommensal animals is presumed to function in fa- cilitat
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