. Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically. ll hsemolyze of a 5 per cent, suspension of sheeps corpusclesin the presence of of fresh guinea-pig serumused as complement. Usually, by the intravenousmethod described, the rabbit can be immunized so thatits serum in 1-3000 dilution will fulfil these re-quirements. To determine the strength of the hemo-lytic serum, three or four days after the third injectionof the rabbit, about 2 of blood are drawn into asmall test-tube from a vein of the rabbits e
. Applied immunology : the practical application of sera and bacterins prophylactically, diagnostically, and therapeutically. ll hsemolyze of a 5 per cent, suspension of sheeps corpusclesin the presence of of fresh guinea-pig serumused as complement. Usually, by the intravenousmethod described, the rabbit can be immunized so thatits serum in 1-3000 dilution will fulfil these re-quirements. To determine the strength of the hemo-lytic serum, three or four days after the third injectionof the rabbit, about 2 of blood are drawn into asmall test-tube from a vein of the rabbits ear. Whenthe blood has clotted and the clear serum separated,the latter is drawn off and the natural complement init destroyed by heating the serum in a water-bath forhalf an hour at 56° C. This deprivation of comple-ment is known as inactivation. The complement usedin titrating the hemolytic amboceptor and in theWassermann test itself is furnished by serum of theguinea-pig, as it has been found that this animal hasan almost constant amount of complement present inits blood. To obtain complement, a full-grown guinea- ff.
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Keywords: ., bookcentury1900, bookdecade1910, bookpublisherphila, bookyear1916
