. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Simple Method of Staining 147 more convenient method is to wipe the glasses as clean as possible with a soft cotton cloth, seize them with fine-pointed forceps, and pass them repeatedly through a small Bunsen flame until it becomes greenish-yellow. The hot glass must then be slowly elevated above the flame, so as to allow it to anneal. This manoeuver removes the organic matter by combustion. It is not expedient to use covers twice for bac- teriologic wor
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Simple Method of Staining 147 more convenient method is to wipe the glasses as clean as possible with a soft cotton cloth, seize them with fine-pointed forceps, and pass them repeatedly through a small Bunsen flame until it becomes greenish-yellow. The hot glass must then be slowly elevated above the flame, so as to allow it to anneal. This manoeuver removes the organic matter by combustion. It is not expedient to use covers twice for bac- teriologic work, though if well cleansed by immer- sion in acid and washing, they may subsequently be employed for ordinary microscopic objects. The fragility of the covers and their likelihood to be broken or dropped at the critical moment, make most workers pre- fer to stain directly upon the slide. The slide should be thoroughly cleaned, and if the material to be examined is spread near one end, the other may serve as a convenient han- dle. The slide is also to be preferred if a number of examinations are to be made simultaneously or for comparison, as it is large enough to contain a number of "; SimpleMethodof Stain- ing.—The material to be examined must be spread in the thinnest possible layer upon the surface of the perfectly clean cover-glass or slide and dried. The most conveni- ent method of spreading is to place a minute drop on the glass with a platinum loop, and then spread it evenly over the glass with the flat wire. Should it be stained at once it would all wash off, so it must next be fixed to the glass by being passed three times through aflame, experience having shown that when drawn through the flame three times the desired effect is usually accomplished. The Germans recommend that a Bunsen burner or a large alcohol lamp be used, that the arm describe a circle a foot in diameter, each revolution occupying a second of time, and the glass being made to pass
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
