. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. 168 CALIFORNIA FISH AND GAME. FIGURE 2. Map of study area, showing stations 1 and 2 on the Santa Clara River. were removed. Samples of eggs were removed at random from each ovary and separated into groups of 150. 100 and 50, while submerged in alcohol to prevent drying. Each of the three samples was dried on absorbant paper prior to weighing to remove excess alcohol. Each sam- ple was weighed on a Mettler balance and the number of egg


. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. 168 CALIFORNIA FISH AND GAME. FIGURE 2. Map of study area, showing stations 1 and 2 on the Santa Clara River. were removed. Samples of eggs were removed at random from each ovary and separated into groups of 150. 100 and 50, while submerged in alcohol to prevent drying. Each of the three samples was dried on absorbant paper prior to weighing to remove excess alcohol. Each sam- ple was weighed on a Mettler balance and the number of eggs per gram computed. An average number of eggs per gram was calculated from the three samples to determine the number of eggs per ovary. The mean egg diameters for December 1968 and February, March and May 1969 were determined by randomly removing 20 ova per fish from the left ovary of several of the largest females. The ova were arranged in a straight line irrespective of size and viewed with a dissecting microscope equipped with an ocular micrometer. Thirty-four specimens from the period of June 1968 to May 1969 were utilized to calculate the seasonal trend in the gonadal-somatic, or ovary weight X 100 maturity index ( ) (James, 1946). The left body weight ovaries were removed, dried for 4 min on absorbent paper, and weighed. The average maturity index for each month was then calcu- lated and plotted against time to show the progressive increase in rela- tive ovary size up to a peak just prior to spawning. Stomach contents were obtained from 48 suckers, 27 to 57 mm, col- lected on October 4, 1968. All specimens were injected with 10% forma- lin and returned to the laboratory for analysis. The posterior limit of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original California. Dept. of Fish and Game; California


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