. Ciba Foundation symposium on drug resistance in micro-organisms: mechanisms of development. Drug resistance in microorganisms. 144 M. J. Thornley, J. Sinai and J. Yudkin proflavine additions were made (Fig. 2). On the other hand, if a growing culture was acidified, growth continued even if all. .o 3 P 2 •o I 60 I20 I80 Time (mins) Fig. 2. Effect of maintaining neutral pH in culture during pro- flavine additions, and of acidifying the culture when large single addition of proflavine made, on growth of Bad. lactis aerogenes. 1. pH maintained neutral throughout incubation, proflavine added
. Ciba Foundation symposium on drug resistance in micro-organisms: mechanisms of development. Drug resistance in microorganisms. 144 M. J. Thornley, J. Sinai and J. Yudkin proflavine additions were made (Fig. 2). On the other hand, if a growing culture was acidified, growth continued even if all. .o 3 P 2 •o I 60 I20 I80 Time (mins) Fig. 2. Effect of maintaining neutral pH in culture during pro- flavine additions, and of acidifying the culture when large single addition of proflavine made, on growth of Bad. lactis aerogenes. 1. pH maintained neutral throughout incubation, proflavine added gradually 2. Culture acidified to pH 4-9, then all proflavine added at once 3. Proflavine added graduaUy 4. No proflavine added gradual proflavine addition 65 ^ proflavine present no proflavine added Time from beginning of proflavine additions. the proflavine was added at once. Finally, acidified fresh medium with the whole amount of proflavine added supported. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation; Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-. Boston, Little, Brown
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