. Contributions from the Botanical Laboratory, vol. 8. Botany; Botany. 212 P1 () w e Mombrant's in the plant cell. I :^i:^ toiioplast is clear, colorless, hyaline, apparently qnite fluid and only slightly sticky. The isolated vacuole swells in a diluted nirdiuni and shrinks in a, concentrated one. If it contains colored sap (as in cells of the beet, occasional cells of Elodea and onion) it is (evident that the pigment is retained. If placed in a colored solution, the dye does not penetrate. Since this layer retains its differential permeability, it seems not unlikely that its physical conditio
. Contributions from the Botanical Laboratory, vol. 8. Botany; Botany. 212 P1 () w e Mombrant's in the plant cell. I :^i:^ toiioplast is clear, colorless, hyaline, apparently qnite fluid and only slightly sticky. The isolated vacuole swells in a diluted nirdiuni and shrinks in a, concentrated one. If it contains colored sap (as in cells of the beet, occasional cells of Elodea and onion) it is (evident that the pigment is retained. If placed in a colored solution, the dye does not penetrate. Since this layer retains its differential permeability, it seems not unlikely that its physical condition may also closely approach that of tlu' same layer in the normal cell. When the tonoplast is elongatplast f(>rms a layer distinct from the swollen mesoplasm, and can easily be separated with the needle. The separation of tonoplast fi'om mesoplasm can b(^ follow(id as an unplasmolyzed proto- plast swells as a I'esult of injury with the needle. Starting at the point of injury, the tonoplast appears to blister (d'f in a manner recalling the separation ot the fertilization membrane from an echinoderin egg. At times, the tonoplast is not. at first, distinct from the swollen mesoplasm, but becomes distinguishable lat(^r as the mesoplasm shrinks away and collapses against the plasmalemma and wall. The tonoplast isohited from unplasmolyzed cells behaves on mani- pulation just as (h>es the same layer from plasmolyzcMl cells. The number of different ways in which separation of the tonoplast can be brought about is strikini'-. If i)lasm(dysis in* deplasmolysis is very lapid the tonoplast m;iy separate from ths^ rest of the cytoplMsm. 11 the protoplast is cut by the i-azor, the tonoplast may sei)arate while the rest of the cytoplasm swells and flows tog(;ther. If an injection oi' the inser- tion of a needle produces too great a strain in the cytoplasm, th:j outer layers may be ruptured or swell, and the tonoplast persist. If the nucleus is injured, the tonoplast will remain distinct
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectbotany, bookyear1892