A treatise on the principles and practice of medicine . rin. It is not proved that it develops a definite toxin. Its resistingpowers are as a rule limited. Drying is endured by the bacillus for six oreven ten months. In decomposing media its average life is one to oneand a half weeks. Heat (106°) kills it in a few minutes. It may survive14° below zero for weeks. Sunlight kills the bacillus in minutes to hours. 1 5 to 20 of sputum are placed in a flask with an equal quantity of 50 per cent,antiformin and the mixture is boiled. Solution of the sputum occurs at once. To 10 the cooled
A treatise on the principles and practice of medicine . rin. It is not proved that it develops a definite toxin. Its resistingpowers are as a rule limited. Drying is endured by the bacillus for six oreven ten months. In decomposing media its average life is one to oneand a half weeks. Heat (106°) kills it in a few minutes. It may survive14° below zero for weeks. Sunlight kills the bacillus in minutes to hours. 1 5 to 20 of sputum are placed in a flask with an equal quantity of 50 per cent,antiformin and the mixture is boiled. Solution of the sputum occurs at once. To 10 the cooled solution (which is sterile and from which cultures or inoculations can bemade) of a mixture of 10 volumes of chloroform and 90 volumes of alcohol areadded and the whole thoroughly shaken. The specimen is centrifugalized about fifteenminutes. Chloroform is found at the bottom of the tube, and on its upper surface thesediment collects. The supernatant fluid is poured off, and with a pipette the sediment istransferred to a glass slide. PLATE V.
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