. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 166 H ZHENG AND T. H DIETZ Table IV The effects of acetazolamide (AZ) and external CI' on Na* transport and net flux of titratable base in salt-depleted Corbicula fluminea; the animals were pre-treated for 12 h before the experiments with AZ added to the bathing solution at a final concentration of mM (no AZ was added to the bathing solution during the experiments) + C1 -c\- F-test Flux -AZ +AZ -AZ + AZ Jn""" ± ± ± ± ir' ± ± ± ±


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 166 H ZHENG AND T. H DIETZ Table IV The effects of acetazolamide (AZ) and external CI' on Na* transport and net flux of titratable base in salt-depleted Corbicula fluminea; the animals were pre-treated for 12 h before the experiments with AZ added to the bathing solution at a final concentration of mM (no AZ was added to the bathing solution during the experiments) + C1 -c\- F-test Flux -AZ +AZ -AZ + AZ Jn""" ± ± ± ± ir' ± ± ± ± h"" ± ± ± ± h" ± ± ± ± tCI ±AZ cr/AZ Data are means and standard errors of measurements for 10 animals and are expressed as fjeq g ' dry tissue h '. J„. net flux; 1,, influx; J„, efflux; superscripts denote flux as either for sodium (Na) or for tilratahle base (B). *: P < , **; P < C]"/AZ: interaction between CP and AZ. in C. fluminea. and that the pathway stimulated by salt depletion is different from that stimulated by 5HT. A Na*/H* exchange mechanism has been considered to be a major pathway for Na* uptake in freshwater bivalves including C. fluminea (McCorkle and Diet/,. 1980; Dietz, 1978, 1985: Henry and Saintsing, 1983; Byrne and Dietz. 1997). However, other Na* uptake pathways, such as indirect coupling to the activity of a H*-translocating- ATPase. may also exist. Recent studies have demon- strated that Na* uptake in the gills of freshwater rainbow trout is energized by the electrogenic H*-ATPase located on the apical membrane of the gill (Lin and Randall. 1995). Serotonin stimulated the CP influx by increasing the active transport component, presumably a CI /HCOr ex-. 8 10 12 J neq g dry tissue h 16 Figure 3. Relationship between CI influx and net loss of titratable base in pondwater-acclimated (PWA) and salt-depleted (SD) Co


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