. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HOMOGENATE RESPIRATION 385 TABLE II Effect of sodium deoxycholate concentration on respiration of homogenized R. pipiens embryos, stage 10-11. Breis and deoxycholate made up separately in M phosphate buffer, pH After equilibration period in respirometer bath, deoxycholate added from side-arm. Final brei con- centration, 20 embryos per ml. Final concentration of sodium deoxycholate, per cent 0 /il. O2 per hour per embryo R. pipiens, DOC-breis. The maximum respiratory rate att
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HOMOGENATE RESPIRATION 385 TABLE II Effect of sodium deoxycholate concentration on respiration of homogenized R. pipiens embryos, stage 10-11. Breis and deoxycholate made up separately in M phosphate buffer, pH After equilibration period in respirometer bath, deoxycholate added from side-arm. Final brei con- centration, 20 embryos per ml. Final concentration of sodium deoxycholate, per cent 0 /il. O2 per hour per embryo R. pipiens, DOC-breis. The maximum respiratory rate attained by plain-breis, , that exhibited by embryos homogenized during the fourth 25-hour period of de- velopment, can be matched by that of deoxycholate-treated breis prepared from em- bryos of any lesser age (Fig. 1). Plain-breis and DOC-breis prepared from em- bryos older than this, however, respire at the same rate. The degree of respiratory elevation obtained earlier is a function of the concentration of DOC, being the optimal concentration (Table II). 5 - 1 - I -. Intatt ... 25 59 ^S 100 hours Development I8*C- FIGURE 2. Respiration of homogenized gastrula-blocked hybrid embryos (R. pipiens $ X R. sylvatica c?). Lower curve for intact embryos constructed from data of Earth (1946) and Moog (1944).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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