. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 330 C. ALEX SHIVERS antibody preparations were mixed with an equal volume of Sorenson's phosphate mixture before analyzing to inaiutaiii equal dilutions in the absorbed and un- antisera. RESULTS sis of antii/cns hi species of RCDHI: Agar-gel diffusion precipitin anal\>is of the antigenic components of egg-jellies .if the four Rumi species revealed similar patterns to the extent that the jelly of each species contained at least Five distinct antigens. One of these antigens was common to all four species. Each of the ^pecie
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 330 C. ALEX SHIVERS antibody preparations were mixed with an equal volume of Sorenson's phosphate mixture before analyzing to inaiutaiii equal dilutions in the absorbed and un- antisera. RESULTS sis of antii/cns hi species of RCDHI: Agar-gel diffusion precipitin anal\>is of the antigenic components of egg-jellies .if the four Rumi species revealed similar patterns to the extent that the jelly of each species contained at least Five distinct antigens. One of these antigens was common to all four species. Each of the ^pecies had a unique combination of cross-reacting antigens and two or three species-specific antigens. The relationships for anti-jelly serum of R. pipicns are This Figure is a drawing of a fully-developed agar diffu- illustrated in Figure 1. /^-^. FIGURE 1. Diagram of double diffusion plate, showing precipitation bands formed by reacting anti-jelly serum of R. pipicns (PA) with the egg-jelly material of R. syk-atica (SJ), R. pipicns CPJ) and R. clainitaus (CJ). a, b and c •= precipitation bands representing compo- nents which are species-specific, shared among the three species and shared between only two of the species, respectively. sion plate in which unabsorbed anti-jelly serum of R. pipicns (Well PA) was reacted with antigens prepared from egg-jelly'material of R. syk'atica (Well SJ), 1\. pipicns ( \\V11 PI i and R. ckunitans (Well CJ). Those components which are present in the jelly of eggs of R. pipicns are represented by lines formed between the anliserum well and well 1 'J (, lines a, b and c. Figure 1). The three lines labelled a are species-specific to R. pipicns. The curvature of the line near< si the aiitisermn well does not represent a cross-reaction between the antiserum and the heterologous jellies. The component common to the egg-jellies of all three species is indicated by the continuous line extending between the- antiscrum well and the three anti-en wel
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
