. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 60 S. W. JEFFREY AND KAZUO SHIBATA \s V^1 CD CJ -§ o in 0. 350 400 500 600 Wavelength in 700 FIGURE 4. Absorption spectrum (curve A) of chlorophyll c in 5:1 methanol-water mixture (pH ) as the control, and the spectrum (curve B) of the same solution at pH mixed with a minute volume of a concentrated NaOH solution and observed 5 minutes after the addition of alkali. Curve C is the spectrum of a different concentration of chlorophyll c in a mixture (pH ) of absolute methanol and 28% aqueous
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 60 S. W. JEFFREY AND KAZUO SHIBATA \s V^1 CD CJ -§ o in 0. 350 400 500 600 Wavelength in 700 FIGURE 4. Absorption spectrum (curve A) of chlorophyll c in 5:1 methanol-water mixture (pH ) as the control, and the spectrum (curve B) of the same solution at pH mixed with a minute volume of a concentrated NaOH solution and observed 5 minutes after the addition of alkali. Curve C is the spectrum of a different concentration of chlorophyll c in a mixture (pH ) of absolute methanol and 28% aqueous ammonia in a volume ratio of 5:1. Curve C was observed 250 minutes after the addition of aqueous ammonia. merit with alkali and were replaced by new bands at 561 and 600 m/j,. Isobestic points existed at 575, 595 and 612 m/t in this transformation. These spectral changes may therefore, be interpreted as due to a simple transformation from one compound to another. The spectral change with ammonia at alkaline pH was found to be different from this change with NaOH. One ml of 28% aqueous ammonia was added to 5 ml of a chlorophyll c solution in methanol, and the spectrum of the mixture was measured at intervals. A different spectral change proceeded slowly during 4 hours of incubation with ammonia at pH , and the resultant spectrum shown by curve C in Figure 4 indicated the Soret band at 426 m/A and three bands at 564, 604, and 668 m/jL, respectively. There seems to be another band near 630 m^, considering the high and flat absorption curve between the two separate bands at 604 and 668 m^,. The two bands at 564 and 604 m/A are similar in position and in the relative height to the visible bands of NaOH-treated chlorophyll c (curve B), so that the band at 668 niju may be characteristic of this ammonia-treated chlorophyll c. The intensification of the Soret band by ammonia was estimated to be times. Please note that these images are extracted from scanned page images that may
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
