. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PERIPHYLIA LUCIFERASE STORED IN OVARY 345 200. pH Figure 6. Effect of pH on the light intensity of the luminescence of coelenterazine catalyzed by luciferases A, B. and C, and on the stability of the same luciferases. The effect on light intensity (solid lines) was measured in 50 mM phosphate buffers, pH or 50 mM Tris-HCl buffers. pH , all containing 1 M NaCl, BSA. and 10 jil of mM methanolic coelenterazine. For measuring the effect on stability (dotted lines), luciferase samples were left standin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PERIPHYLIA LUCIFERASE STORED IN OVARY 345 200. pH Figure 6. Effect of pH on the light intensity of the luminescence of coelenterazine catalyzed by luciferases A, B. and C, and on the stability of the same luciferases. The effect on light intensity (solid lines) was measured in 50 mM phosphate buffers, pH or 50 mM Tris-HCl buffers. pH , all containing 1 M NaCl, BSA. and 10 jil of mM methanolic coelenterazine. For measuring the effect on stability (dotted lines), luciferase samples were left standing for 30 min at room temperature, in ml of a solution having various pH values, then luminescence intensity was measured under the standard condition (pH ). The solutions used were M HC1. M HC1. M acetic acid, 50 mM phosphate, 50 mM Tris-HCl, M NH3, and M NaOH. all containing 1 M NaCl and BSA. The amount of luciferases used for measuring each point were luciferase A, 150 LU; luciferases B and C. 170 LU. this organism. The luciferase occurs in a soluble form (luciferase L) and also as an insoluble aggregate. The sol- uble form is responsible for the in vivo bioluminescence of the animal and is distributed widely, not only in the epithe- lial photocytes but also in the mesoglea of the large coronal dome. The insoluble form exists in the paniculate matter distributed abundantly in the ovary, particularly in the eggs. The size of the particles, measured by differential filtration, was larger than /urn and smaller than 2 /urn; the actual size is probably close to the low end of this range according to previous microscopic observation (Flood et al., 1996). Like soluble luciferase L, the paniculate matter is highly active in catalyzing the luminescence of coelenterazine, but its involvement in the in vivo bioluminescence is uncertain. The luciferase activity of particulate matter is increased several times by solubilization using 2-mercaptoeth
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
