. Banana wilt. Fusarium wilt of banana. 1919] Brandes: Banana Wilt 371 mycelium growing on the surface of rotted roots was proved, upon cul- tivation to be the Fusarium used for inoculum. Cotton seedlings three weeks old were next inoculated with macro- conidia by making longitudinal slits in the hypocotyl with a sharp scalpel and inserting the inoculum with a platinum needle. The same number of plants and the same strains of Fusarium were used for this experi- ment as for the preceding. The results were not so uniform, still they were convincing. Where an abundance of inoculum was used the pl
. Banana wilt. Fusarium wilt of banana. 1919] Brandes: Banana Wilt 371 mycelium growing on the surface of rotted roots was proved, upon cul- tivation to be the Fusarium used for inoculum. Cotton seedlings three weeks old were next inoculated with macro- conidia by making longitudinal slits in the hypocotyl with a sharp scalpel and inserting the inoculum with a platinum needle. The same number of plants and the same strains of Fusarium were used for this experi- ment as for the preceding. The results were not so uniform, still they were convincing. Where an abundance of inoculum was used the plants wilted and died in from one to two days (text fig. 5). The stems above and to a lesser extent below the wounds became discolored and finally rotted. Where a small amount of inoculum was used the plants were undeniably injured, as was made evident by a retarded development, but they seemed to recover and resumed growth. The control plants,. Fig. 4. Cotton Growing in Soil Inoculated with Fusarium cubense (left) Cotton Growing in Sterilized Soil (right) which had been wounded in exactly the same manner with a sterile scalpel were all half again as tall as the largest of the inoculated plants one week after inoculation. Only one of the plants inoculated with F. vasinfectum showed typical symptoms of cotton wilt. The vascular bundles became discolored, and the organism was reisolateci from a leaf petiole. Fungus hyphae were determined to be present in the brown, injured petioles of plants copiously inoculated with F. cubense also. It is to be regretted that the results of this experiment were not more decisive. The writer believes, however, that certain conclusions may be drawn from this evidence, when it is supplemented with the results ob- tained (p. 365) in the experiments on the effect of by-products of the fungus on plants selected at random from the vegetable kingdom. The fact that pathologic symptoms were produced when the banana organism was copiously inoculated into co
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