. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson 172 Cytoplasm 1 nels of other varieties of maize as well as in the seeds of other grains (barley, wheat, rye, oats). Aleurone grains stain deeply with mitochondrial techniques which do not at all stain the liquid vacuoles from which they are derived, except in periods preceding solidification when the stains bring about flocculation of the col- loidal solution, and the formation in the vacuoles of precipitates stainable with iron haematoxylin. At the time of germination, when the seed again takes up
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilliermond - Atkinson 172 Cytoplasm 1 nels of other varieties of maize as well as in the seeds of other grains (barley, wheat, rye, oats). Aleurone grains stain deeply with mitochondrial techniques which do not at all stain the liquid vacuoles from which they are derived, except in periods preceding solidification when the stains bring about flocculation of the col- loidal solution, and the formation in the vacuoles of precipitates stainable with iron haematoxylin. At the time of germination, when the seed again takes up water, the aleurone grains absorb it and become semi-fluid. At this period they often show again a tendency to elongate into filaments capable of anastomosing in a network. At the beginning of hydration, the aleurone grains stain deeply and homogeneously with vital dyes. Then the filamentous and semi-fluid, reticulate vacuoles derived from them swell as water continues to be taken in and appear as spherical liquid vacuoles which by coales- cence gradually become transformed into large vacuoles in which the vital dyes cause strongly colored precipitates. In sections prepared with mitochondrial techniques, the forms which were reticulate and filamentous, at the beginning of ger- mination, show a heavily stained, compact contour which is filamentous, or granular, and which is surrounded by a clear zone. ^^ W I '^^^ spherical liquid vacuoles, which succeed •15^ I * I them, still enclose stained corpuscles but these corpuscles become less and less abun- dant, as more and more water is taken into the vacuoles and, finally, in the large vacu- oles, no stained contents are found. ':-\. •A. Fig. 119. — Tulip. Epi- dermal cells of petals of a dark red variety. Frag- mentation of large vacuole, containing anthocyanin, into small, sometimes reticulate, or filamentous, vacuoles caused by plasmolysis in a 5% solution of NaCl. Reversibility of form in the vacuolar sys- tem. — Pierre
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