. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Chapter VII 67 — The Chondriome they stain selectively with Janus green, methyl violet 5B and Dahlia violet. Recent research (Guilliermond and Gautheret) has made known other vital dyes for staining chondriosomes: gentian violet, crystal violet, Hoffman violet, methyl green, iodine green, malachite green and Victoria blue. Among these, Janus green is one of the least toxic. Used with Saprolegnia in weak concentrations ( solutions) it stains only the chon- driosomes, giving them a bluish green color in filament
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Chapter VII 67 — The Chondriome they stain selectively with Janus green, methyl violet 5B and Dahlia violet. Recent research (Guilliermond and Gautheret) has made known other vital dyes for staining chondriosomes: gentian violet, crystal violet, Hoffman violet, methyl green, iodine green, malachite green and Victoria blue. Among these, Janus green is one of the least toxic. Used with Saprolegnia in weak concentrations ( solutions) it stains only the chon- driosomes, giving them a bluish green color in filaments which continue to show strong cytoplasmic currents. Staining is there- fore clearly vital. This vital staining of the chondriosomes by Janus green is only transitory. It is observed that at the end of a few moments the chondriosomes lose their green color, whereas a rose tint appears in the vacuolar system. These ob- servations are explained by the fact that the chondriosomes re- duce Janus green to its rose derivative and this latter, having more affinity than its oxidized form ^ for the vacuolar system, diffuses i \^\/. • into it. If higher concentrations of luj^/"^^) \ ^ Janus green are used, it stains not j('\\(^,^^W^H-•^ only the chondriosomes but also the b'^XlljKv^ vacuolar system. The chondriosomes i \\f^ /3^^ then remain colored but the filaments i[ 4\^> "pf '^i die rapidly and do so generally with- out reducing the dye. At concen- trations above the dye stains the chondriosomes at first but rapid- ly causes them to become vesiculate, then accumulates in the vacuolar ^ „ „ , , ^, ,, 1 1 j_ 1 • Fig. 32. — Portions of a filament of system as well, and later brmgS SavroUgnia, showing the similarity of about the death of the fungus. Most J^^) uying and (B) fixed tissue, in , the latter the hpide granules are of the other dyes, among them not visible, c, chondriosomes; cg, hp- methyl violet and Dahlia violet, are i^^^hoT""'^'' ^
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