. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 256 RAVENNA UKELES Platymonas sp., Chlamydomonas sp. of the order Yolvocales; Chlorococcum sp., Chlorella sp. of the order Chlorococcales; and Protococcus of the order Chaeto- phorales. Temperature requirements of the pennate diatoms, Nitzschia laci'is, Amphiprora sp. and Amphora sp., and of the centralean diatom, Actinocyclus sp., were also investigated. Glass-covered, 25-milliliter Fernbach flasks were used as culture vessels. These flasks allow for complete illumination and an adequate surface-volume relationship. Each ve
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 256 RAVENNA UKELES Platymonas sp., Chlamydomonas sp. of the order Yolvocales; Chlorococcum sp., Chlorella sp. of the order Chlorococcales; and Protococcus of the order Chaeto- phorales. Temperature requirements of the pennate diatoms, Nitzschia laci'is, Amphiprora sp. and Amphora sp., and of the centralean diatom, Actinocyclus sp., were also investigated. Glass-covered, 25-milliliter Fernbach flasks were used as culture vessels. These flasks allow for complete illumination and an adequate surface-volume relationship. Each vessel contained 10 ml. of medium and was inoculated with ml. of a bacteria-free unialgal stock culture. Stock cultures from which the inocula were made are maintained at ° C. ± 1° C. in a light room. The basal medium consisted of the following: KH.,PO4, 20 mg.; thiamine HC1, mg.; biotin, 2 ; vitamin B-12, 2 /ig.; XaXO.,, 150 mg.; ferric sequestrene (13% Fe), 10 mg.;. FIGURE 1. Constant temperature apparatus, showing four of the six individual baths, the refrigerating system, the lighting system and the culture vessels. NH4C1. 50 mg.; CuSO4-5H,O, mg.; ZnSO4-7H.,O, mg.; CoCV6H2O, mg.; MnQ2-4H,O. mg.; Xa,MoO4-2H,O. mg.; sea water, 1 liter (22 to 27 ppt). XH4C1 was eliminated from the basal medium for /. galbana. Experimental temperatures were maintained in a series of constant temperature baths regulated to ± ° C. The culture flasks were submerged on plastic mesh racks into the water baths and incubated at temperatures ranging from 8° C. to 39° C. The apparatus allows for the determination of the effects of six tempera- tures simultaneously. Light was received from a bank of cool fluorescent bulbs suspended over the water bath to give maximum illumination with a minimum amount of heat (Fig. 1). At periodic intervals cultures were observed for. Please note that these images are extracted from scanned page images that may have
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
